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Research tools

Reconstitution calculator

Work out the concentration of a reconstituted research solution and the volume that contains a target quantity. A laboratory measurement aid — for research use only.

Reconstitution calculator

Solution concentration mg/mL ( mcg/mL)
Volume for target mL ( units)

The target volume exceeds the solvent added — increase the solvent volume or lower the target quantity.

For laboratory measurement and research use only. This tool reports the concentration of a reconstituted solution; it is not dosing guidance and the products are not for human or veterinary use. “Units” refer to graduations on a standard measuring syringe.

How the calculation works

Reconstitution is the laboratory process of dissolving a lyophilised (freeze-dried) peptide in a suitable solvent — commonly bacteriostatic or sterile water — so it can be measured and studied. The resulting concentration is simply the mass of peptide divided by the solvent volume:

concentration (mg/mL) = peptide mass (mg) ÷ solvent volume (mL)

To find how much solution contains a target quantity, divide the target (in mcg) by the concentration (in mcg/mL). Multiplying that volume by the syringe rating gives the equivalent number of graduations on a standard measuring syringe (a U-100 syringe has 100 units per millilitre).

volume (mL) = target (mcg) ÷ concentration (mcg/mL)
units = volume (mL) × syringe rating

A worked example

Suppose a vial contains 10 mg of peptide and you add 2 mL of solvent. The concentration is 10 ÷ 2 = 5 mg/mL, or 5,000 mcg/mL. A target quantity of 250 mcg is contained in 250 ÷ 5,000 = 0.05 mL, which is 5 units on a U-100 measuring syringe.

Frequently asked questions

Once reconstituted, peptides are generally less stable and are typically kept refrigerated and used within a shorter window. Follow standard laboratory handling practice for the specific compound.
Bacteriostatic or sterile water is commonly used in laboratory settings, though the appropriate solvent depends on the peptide. We offer bacteriostatic water as a research supply.
Reconstitution is the laboratory process of dissolving a lyophilised (freeze-dried) peptide in a suitable solvent, such as bacteriostatic water, before it is used in research.